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monoclonal anti ifn lambda 3 antibody  (R&D Systems)
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R&D Systems monoclonal anti ifn lambda 3 antibody
Figure 5. Production of IFN <t>lambda-3</t> in Arabidopsis seeds. (a) Schematic representation of recombinant IFN lambda-3 fused with 3′12S1. IFN lambda-3CDS represents the coding sequence of the IFN lambda-3 gene. 6 × His- HDEL represents the ER-retention signal (HDEL) fused with a 6 × Histidine tag. (b) CBB staining of seed proteins from WT, 12s1.4, and four independent transgenic lines of IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4. The arrowhead indicates IFN lambda-3 bands. (c) Immunoblot analysis of seed protein from WT, 12s1.4, and IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4 plants. (d) Relative activity of purified recombinant IFN lambda-3. IFN lambda-3 activities are relative to the activity of recombinant IFN lambda-3 produced in human cells. Values are presented as the mean ± SE of four independent experiments.
Monoclonal Anti Ifn Lambda 3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
monoclonal anti ifn lambda 3 antibody - by Bioz Stars, 2026-02
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ifn λ3  (R&D Systems)
93
R&D Systems ifn λ3
Figure 5. Production of IFN <t>lambda-3</t> in Arabidopsis seeds. (a) Schematic representation of recombinant IFN lambda-3 fused with 3′12S1. IFN lambda-3CDS represents the coding sequence of the IFN lambda-3 gene. 6 × His- HDEL represents the ER-retention signal (HDEL) fused with a 6 × Histidine tag. (b) CBB staining of seed proteins from WT, 12s1.4, and four independent transgenic lines of IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4. The arrowhead indicates IFN lambda-3 bands. (c) Immunoblot analysis of seed protein from WT, 12s1.4, and IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4 plants. (d) Relative activity of purified recombinant IFN lambda-3. IFN lambda-3 activities are relative to the activity of recombinant IFN lambda-3 produced in human cells. Values are presented as the mean ± SE of four independent experiments.
Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ifn λ3/product/R&D Systems
Average 93 stars, based on 1 article reviews
ifn λ3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti ifn λ3  (R&D Systems)
93
R&D Systems anti ifn λ3
Figure 5. Production of IFN <t>lambda-3</t> in Arabidopsis seeds. (a) Schematic representation of recombinant IFN lambda-3 fused with 3′12S1. IFN lambda-3CDS represents the coding sequence of the IFN lambda-3 gene. 6 × His- HDEL represents the ER-retention signal (HDEL) fused with a 6 × Histidine tag. (b) CBB staining of seed proteins from WT, 12s1.4, and four independent transgenic lines of IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4. The arrowhead indicates IFN lambda-3 bands. (c) Immunoblot analysis of seed protein from WT, 12s1.4, and IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4 plants. (d) Relative activity of purified recombinant IFN lambda-3. IFN lambda-3 activities are relative to the activity of recombinant IFN lambda-3 produced in human cells. Values are presented as the mean ± SE of four independent experiments.
Anti Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ifn λ3/product/R&D Systems
Average 93 stars, based on 1 article reviews
anti ifn λ3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

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Figure 5. Production of IFN lambda-3 in Arabidopsis seeds. (a) Schematic representation of recombinant IFN lambda-3 fused with 3′12S1. IFN lambda-3CDS represents the coding sequence of the IFN lambda-3 gene. 6 × His- HDEL represents the ER-retention signal (HDEL) fused with a 6 × Histidine tag. (b) CBB staining of seed proteins from WT, 12s1.4, and four independent transgenic lines of IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4. The arrowhead indicates IFN lambda-3 bands. (c) Immunoblot analysis of seed protein from WT, 12s1.4, and IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4 plants. (d) Relative activity of purified recombinant IFN lambda-3. IFN lambda-3 activities are relative to the activity of recombinant IFN lambda-3 produced in human cells. Values are presented as the mean ± SE of four independent experiments.

Journal: Scientific reports

Article Title: Fusing the 3'UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds.

doi: 10.1038/s41598-023-39356-3

Figure Lengend Snippet: Figure 5. Production of IFN lambda-3 in Arabidopsis seeds. (a) Schematic representation of recombinant IFN lambda-3 fused with 3′12S1. IFN lambda-3CDS represents the coding sequence of the IFN lambda-3 gene. 6 × His- HDEL represents the ER-retention signal (HDEL) fused with a 6 × Histidine tag. (b) CBB staining of seed proteins from WT, 12s1.4, and four independent transgenic lines of IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4. The arrowhead indicates IFN lambda-3 bands. (c) Immunoblot analysis of seed protein from WT, 12s1.4, and IFN lambda-3–6 × His-HDEL-3′12S1/12s1.4 plants. (d) Relative activity of purified recombinant IFN lambda-3. IFN lambda-3 activities are relative to the activity of recombinant IFN lambda-3 produced in human cells. Values are presented as the mean ± SE of four independent experiments.

Article Snippet: Monoclonal anti- IFN lambda-3 antibody (MAB5259, R&D Systems, Minneapolis, USA) was used in the immunoblot analysis.

Techniques: Recombinant, Sequencing, Staining, Transgenic Assay, Western Blot, Activity Assay, Purification, Produced